LOCUS       40924_49227        4588 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD049, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 4588)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4588)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..410
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    408..2303
                     /note="CVD-CYA; device for the GC-adaptor assembly: 
                     cyanobacterial replicons"
     misc_feature    408..428
                     /note="G5C5; GC-adaptor for seamless assembly, flanking 
                     functional devices, custom sequences or cyanobacterial 
                     replicons; synthetic"
     misc_feature    429..434
                     /label=XbaI
                     /note="XbaI"
     CDS             585..1619
                     /codon_start=1
                     /product="repA"
                     /label=repA
                     /note="repA-pDC1; derived from pSUN202; Nostoc sp. PCC8009"
                     /protein_id="AII99715.1"
                     /translation="MANQNRAATQVEGLNHCPLTSQEDRSALSSNVPLPLVLILVKAEI
                     ILQPFMVRLHSGISRQPWARLWYLLRGFDDGSGRVKEIPLVLVEEILGVSQATVYRWLD
                     QGKAVGAFLSGYKVRIGLLTVYLGGLTKVAWHLNLKDWGVVAECPLWEVNANIRALTTG
                     IVTQRLQQRSRYAANRKLKPEYRKSYGAPHPNELLGDEGQSSFKLGAGEVPFVLHVSPS
                     RVFVSKSFIHYGTSQNAVSCKLGIHTRTVRRHQRTLGMEKRQLCQAKYEYAQLDRAVSN
                     EASECWAWSSEGTQSDIGYQSLGISHWVKGRSRFLMASCQEPESSSRTLTSCQRANCLG
                     VSSK"
     misc_feature    991..998
                     /label=SwaI
                     /note="SwaI"
     misc_feature    2277..2282
                     /label=MfeI
                     /note="MfeI"
     misc_feature    2283..2303
                     /note="C3G3; GC-adaptor for seamless assembly, flanking 
                     cyanobacterial replicons or Escherichia coli origins to be 
                     assembled with a cyanobacterial replicon; synthetic"
     misc_feature    2301..2306
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    2325..2330
                     /label=XbaI
                     /note="XbaI"
     primer_bind     complement(2367..2383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2391..2407)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2415..2445)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2460..2481)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2769..3357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3531..4388)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4389..4493)
                     /label=AmpR promoter
     misc_feature    4519..4524
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgaggatatc gggggccccc
      421 ggggggattc tagactgggc aatcattcct taatcaccca gaagcaatga acttcaaaca
      481 tattaccacc cttcgaggat cagcacagta ttcagtcgag cggaagcgaa aacttaaatt
      541 tagtgaagcg attggcacat tgaaccaggt ttgagtgaaa accagtggcc aatcaaaatc
      601 gcgcagctac ccaagttgag gggctgaacc attgccccct gacttcacag gaggatcgtt
      661 cagccttaag ttcaaatgta ccactcccgt tggttttgat actagtcaaa gccgagataa
      721 ttttgcagcc gttcatggtg cggttacaca gtggaattag ccgtcaacct tgggcgcggc
      781 tgtggtattt gttgcgcggt tttgatgatg ggagcggacg ggttaaagaa attccgctcg
      841 tgctggtaga ggagatattg ggagttagtc aagccactgt gtacagatgg cttgaccagg
      901 gaaaagcagt aggggcattt ttgtcgggat acaaggtaag aattggactg ctcacggttt
      961 acctgggcgg actgactaag gtggcttggc atttaaattt aaaagattgg ggggtagtag
     1021 cagaatgtcc gttatgggag gtcaacgcca acattcgagc gctgaccacg ggcatagtca
     1081 cacagcgttt gcaacaaaga agtagatacg cggctaaccg caaactgaaa ccggagtatc
     1141 gcaagtctta cggagcgcca catccaaacg aactgttggg ggatgagggg caatcctcgt
     1201 ttaaattggg agcgggggaa gtaccttttg ttcttcatgt cagtccgtcg cgggtgttcg
     1261 tttctaaaag cttcattcat tacgggactt ctcaaaatgc ggtcagctgt aaattgggca
     1321 tccacacgcg cacagtgcgg cggcatcagc gaacgctcgg catggagaaa aggcaactgt
     1381 gccaagcgaa gtatgaatac gctcaacttg accgggctgt aagcaatgaa gcgtctgagt
     1441 gctgggcttg gagtagcgag ggcactcagt cagacattgg gtatcagtca ttgggtatca
     1501 gtcattgggt gaaggggcga tcgcgttttc tgatggcatc ctgccaggag ccagaaagca
     1561 gcagccgaac acttaccagc tgccagcggg cgaactgtct gggcgtttct tccaaatgaa
     1621 gggtaaaacc tggatggctc gctgcaacat ttaccgcgag caattcaagc taacaaccat
     1681 gcgcgcaaag cggcggaagt ggcgttgtaa actggcacag tgccagtttc acgaaaatcg
     1741 agccgtcccg caggcagacg attgcaaagc aatcatcaca agccccagga agtgacggga
     1801 catttttgag aatttttttg agggggtaag atgtgggttt tgaagatgct ggtagaacat
     1861 gagttggatg aagaaaacca ggtagtctgg acatgtgatg agctatgctc tggcagtctg
     1921 cgccaatgca tggatcatgc tgcttcccta gtgccgaaat atcgcggatg gggattaact
     1981 ttaaattcca ttgcagaagc ttggaatgac atattttgtc aaaaccttaa gagtgcgtgg
     2041 cttgatgcca aacgcgaaaa tttttttgta caacgagttc actaggcact tttcgcaaca
     2101 gttgaaactt tcagccaaaa ctggcgtact gtgcgtcaaa atttgctggc ggataacata
     2161 aggttgtccg gtaaattttt ggaagttttt actggacaag tgaacttctc cggttgcaaa
     2221 ttttggtaaa tgcatcggca ataagtatac taatcggcac gagacgcgat tgaaaacaat
     2281 tgatccccgg gcccgggccc gatatcctcg gtacccgggg atcctctaga gtcgacctgc
     2341 aggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     2401 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2461 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2521 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2581 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2641 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2701 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2761 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2821 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2881 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2941 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3001 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3061 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3121 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3181 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3241 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3301 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3361 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3421 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3481 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3541 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3601 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3661 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3721 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3781 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3841 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3901 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3961 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4021 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4081 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4141 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4201 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4261 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4321 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4381 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4441 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4501 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     4561 aataggcgta tcacgaggcc ctttcgtc
//
